CRISPR-Cas9 enables rapid gene editing applications

Cas9 protein is the main workhorse for genome editing. Target is recognized with small guide RNA molecule by direct base pairing with DNA sequence. Additionally, short DNA sequence string called PAM is recognized directly by Cas9 protein. Once Cas9 binds to the target and the R-loop is formed, two active sites cleave opposite DNA strands and forms dsDNA break. 

Cas9 target selection is limited by PAM:

• AT rich genomes
• Targeting single allele
• Base editing

Novel Cas9 proteins and other Cas proteins further expands the toolbox

Cas9 orthologs recognizes different PAMs and have different properties (size, thermostability, cleavage pattern).

Learn more about Caszyme technology here.

Caszyme characterization pipeline: